The Textbook of Biochemistry is aimed to serve as a complete textbook for the second-semester students of Bachelor in Pharmacy covering theoretical parts as per the latest syllabus prescribed by the Pharmacy Council of India, New Delhi (Course code BP 203T). This book will also help the B. Pharm students who are preparing for the GPAT Exam. It will prove to be very useful not only for the students pursuing Bachelor in Pharmacy but also for the students of other paramedical courses like a Bachelor in Physiotherapy, D. Pharm, Diploma in Optometry, B.Sc Nursing, etc., considering that Biochemistry has become the main subject of all the courses in Medical and allied health sciences. The main objective of this book is to provide students with knowledge of the catalytic role of enzymes, the importance of enzyme inhibitors in the design of new drugs, and the therapeutic and diagnostic applications of enzymes. Upon completion of the study of this book, students will be able to understand the metabolism of nutrient molecules in physiological and pathological conditions. This book will also help to understand the genetic organization of the mammalian genome and the functions of DNA in the synthesis of RNAs and proteins. This book is a comprehensive treatment of various metabolic pathways and the importance of biomolecules. This book strikes a balance between essential and advanced areas of knowledge apart from general topics. This book will be beneficial for the students as well as the professors, as it contains complete theoretical parts as per the syllabus.