This comprehensive text on Python programming is designed for undergraduate and postgraduate students in Computer Science and Information Technology. Whether you are a beginner or have limited programming knowledge, this book offers a structured learning experience, starting with foundational concepts and progressing to complex topics like machine learning. Divided into three parts, the book ensures a smooth progression from the basics and core concepts of Python to machine learning with Python. It covers fundamental topics such as data types, variables, operators, and interactive input/output, enabling readers to write simple yet effective Python programs. Subsequently, the text explores advanced concepts such as control flow, functions, file handling, object-oriented programming, modules, and data visualization through graph plotting, empowering readers to develop robust and complex Python applications. Finally, the book introduces readers to the world of machine learning, covering essential topics like data preprocessing, supervised and unsupervised learning, and algorithm implementation. The book equips students to excel in Python programming and seamlessly transition into machine learning, enabling them to design and implement customized algorithms for their datasets.