This book is aimed to serve as a complete practical book for Pharmacy students (D and B. pharm students). This book will prove to be very useful not only for the students pursuing Pharmacy students but also for the students of other paramedical courses like a Bachelor in Physiotherapy, Diploma in Optometry, BSc Nursing, etc. The main objective of this book is to provide students with practical knowledge of the catalytic activity of enzymes and the importance of temperature which affects enzyme activity. Upon completion of the all experiments in this book, students will be able to understand the identification tests for carbohydrates, proteins, and lipids. This book will also help to understand the characterization of DNA. This book is a comprehensive practical knowledge of various biomolecule markers determination in the blood such as cholesterol, HDL-C, triglycerides, and liver function test enzymes SGOT and SGPT. This book will be beneficial for the students as well as the professors and researchers.